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| Title: | VARIATION IN THE PROTEIN LEVEL OF DIFFERENT ACCESSIONS OF PIGEON PEA (Cajanus cajan L. Millspaugh) |
| Authors: | OLAIYA, Aderonke Eunice
Agbolade, J. O. |
| Keywords: | ACCESSIONS
(Cajanus cajan L. Millspaugh)
VARIATION
PROTEIN LEVEL
PIGEON PEA |
| Issue Date: | Oct-2015 |
| Publisher: | FEDERAL UNIVERSITY OYE EKITI |
| Series/Report no.: | DEPARTMENT OF BIOTECHNOLOGY PROJECT;BTH/11/0257 |
| Abstract: | Ten accessions of Pigeon pea (Cajanus cajan) obtained from National Centre for
Genetic Resources and Biotechnology (NACGRAB), Ibadan, Oyo state, were
assessed for their genetic and phylogenic relatedness through electrophoretic
analysis of the seed proteins. 0.2g of the seeds were weighed and macerated with
mortar and pestle in 0.2M phosphate buffer containing 0.133M of acid (NaH2PO4)
and 0.067 of base (Na2HPO4) at Ph 6.5.Protein characterization with standard
marker revealed that the seeds of the 10 accessions contained proteins (B.S.A,
Oval Albumin, Pepsinogen, Trypsinogen and Lysozyme) with molecular weights
ranging from 66 and above kda, 45 – 65 kda, 44 – 33 kda, 32-24 kda and 23-14 kda
respectively. |
| Description: | All the accessions had at least two proteins and two major bands in
common. The study revealed intraspecific similarities and genetic diversity in
protein contents among the ten accessions of pigeon pea (Cajanus cajan). |
| URI: | http://repository.fuoye.edu.ng/handle/123456789/1355 |
| ISSN: | BTH/11/0257 |
| Appears in Collections: | Department of Plant Science Thesis
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