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|Title: ||Acute and sub-chronic toxicity proﬁle of methanol leaf extract of Gouania longipetala in rats|
|Authors: ||Asuzu, I. U|
Maxwell, I. Ezeja
Aruh O., Anaga
|Keywords: ||Gouania longipetala Toxicity Hematology Histopathology|
|Issue Date: ||31-Oct-2013|
|Publisher: ||Elsevier Science|
|Abstract: ||Ethnopharmacological relevance: Gouania longipetala leaves are commonly used in folkloric medicine in
Africa and other parts of the world for treatment of edema, febrifuges, veneral diseases, lumbago, heart
diseases, diabetes mellitus malaria, etc. This study therefore evaluated safety proﬁle of the methanol leaf
extract of the plant using acute and sub-chronic studies in rat model.
Materials and methods: Acute toxicity test of the plant lasted for 48 h with oral administration of graded
doses (100–4000 mg/kg) of Gouania longipetala extract (GLE) in rats. The rats were observed for signs of
toxicity and death. The sub-chronic toxicity was evaluated by administration of different doses (2.5, 5 and
10 mg/kg) of GLE daily in feed for 90 days. On days, 30, 60 and 90, blood samples collected from the
retro-orbital plexus of the eye of the rats were used for evaluation of serum biochemistry, hematology,
lipid peroxidation and in vivo antioxidant activities. Histopathological evaluations of the kidney, liver,
lungs and heart were also done.
Results: The acute toxicity test revealed no observable signs of toxicity or morbidity. Sub-chronic study
showed that GLE signiﬁcantly (po0.05) increased relative liver weight on day 90 at 10 mg/kg. There
were no signiﬁcant variations in the hematological parameters of both GLE treated and untreated rats.
The extract signiﬁcantly (po0.05) reduced total cholesterol, triglycerides, very low density lipoproteins
and increased high density lipoproteins which was more prominent on day 90 at the dose of 10 mg/kg.
The extract signiﬁcantly (po0.05) increased liver enzyme markers at the doses used. GLE also
signiﬁcantly (po0.05) increased serum urea at the dose of 10 mg/kg on day 90. The extract caused
dose-dependent and signiﬁcant (po0.05) increase in superoxide dismutase and decrease in malondiadehyde.
Histopathological studies revealed degenerative changes in the kidney and liver.
Conclusion: The results of the study suggest that Gouania longipetala is well tolerated in short term
therapies, but may have long term toxic effects on the kidney and liver. firstname.lastname@example.org|
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