Development of extraction protocol for phenolic compounds in musk tree seed (Buchholzia coriacea)

Abstract

The impact of extraction methodology and polarity of extraction solvents on the assay of phenolic compounds was investigated using musk tree seed (Buchholzia coriacea) as a model substrate. The efficiency of four solvents (methanol, ethanol, acetone and ethyl acetate) at 20, 40, 60, 80 and 100% aqueous solution and five extraction methods (shaking, vortex mixing, stirring, sonication, sonication/vortexing and accelerated solvent extractor) in extracting the phenolics of B. coriacea were evaluated. Extracts were analyzed for phenolic content by high-performance liquid chromatography and Folin–Ciocalteu assays. Two major phenolic peaks (1 and 2) were isolated from the B. coriacea seed extract in the HPLC which were suspected to be lupeol and β-sitosterol. Methanol at 20% aqueous solution was found to be most efficient in extracting the total phenolic and HPLC peak 1 component of B. coriacea whereas acetone at 80% aqueous solution was more efficient in extracting peak 2 component. On the other hand Accelerated Solvent Extractor (ASE) extraction procedure was noted to be most efficient for extracting the total phenolics and HPLC peak 2 components while sonicator/vortexing was more efficient in extracting peak 1 component.