http://repository.fuoye.edu.ng:80/handle/123456789/804 2026-04-16T04:42:07Z http://repository.fuoye.edu.ng:80/handle/123456789/1355 Title: VARIATION IN THE PROTEIN LEVEL OF DIFFERENT ACCESSIONS OF PIGEON PEA (Cajanus cajan L. Millspaugh) Authors: OLAIYA, Aderonke Eunice; Agbolade, J. O. Abstract: Ten accessions of Pigeon pea (Cajanus cajan) obtained from National Centre for Genetic Resources and Biotechnology (NACGRAB), Ibadan, Oyo state, were assessed for their genetic and phylogenic relatedness through electrophoretic analysis of the seed proteins. 0.2g of the seeds were weighed and macerated with mortar and pestle in 0.2M phosphate buffer containing 0.133M of acid (NaH2PO4) and 0.067 of base (Na2HPO4) at Ph 6.5.Protein characterization with standard marker revealed that the seeds of the 10 accessions contained proteins (B.S.A, Oval Albumin, Pepsinogen, Trypsinogen and Lysozyme) with molecular weights ranging from 66 and above kda, 45 – 65 kda, 44 – 33 kda, 32-24 kda and 23-14 kda respectively. Description: All the accessions had at least two proteins and two major bands in common. The study revealed intraspecific similarities and genetic diversity in protein contents among the ten accessions of pigeon pea (Cajanus cajan). 2015-10-01T00:00:00Z http://repository.fuoye.edu.ng:80/handle/123456789/1347 Title: ESTABLISHING A METHOD FOR THE MASS PROPAGATION OF Piper guineense (Schumach) VIA TISSUE CULTURE Authors: Ogechi Tope, OKAFOR; PROF. S.V.A., UZOCHUKWU Abstract: Piper guineense (Schumach) has great potentials for economic exploration because of the proven use of its medicinal content in human health. The plant material is usually obtained from thick forests by women who have to walk long distances in the bush to reach it. A technique that improves its propagation and domestication, such as tissue culture, becomes necessary. This study was therefore initiated to develop a method for the mass propagation of Piper guineense seedlings using in vitro regeneration. Plants react differently to media concentrations and constituents for their in vitro regeneration. Comparative growth of Piper guneensee inoculated on Murashige and Skoog(MS) medium supplemented with some growth regulators were investigated. Mature nodes were collected from the medicinal garden of National Centre for Genetic Resources and Biotechnology (NACGRAB) Ibadan. Nodes of the species were inoculated onto MS media. Description: The MS media was supplemented with the following concentrations of growth regulators 0.25, 0.50, 0.75, 1.0mg/l. BAP, the same concentration for KIN and 0.05mg/l of NAA for all replicate. After two weeks of inoculation it was observed that, the plant let with BAP 0.50mg/l +NAA 0.05mg/l were found contamination-free and sprouted, while other treatments showed contamination including control. Furthermore, after 7 days the sprouted, plantlets with treatment of BAP 0.50mg/l +NAA 0.05mg/l were also contaminated, thereby yielding no positive result. Many repeats of the experiment also yielded contaminated products. 2015-10-01T00:00:00Z http://repository.fuoye.edu.ng:80/handle/123456789/1335 Title: COMPARATIVE STUDY OF VEGETATIVE AND FRUIT MOPHOLOGY OF FOUR VARIETIES OF OKRA (Abelmoschus escululentus(L.) moench) Authors: MONSURAT OMOTOLA, IYIOLA; Prof. B.O., Akinyele Abstract: Comparative study of vegetative and fruit morphology of four varieties of okra (Abelmoschus esculentus (L.)Moench was investigated in the screen house of the Department of plant science and biotechnology, Federal University of Oye-Ekiti. Two seeds of each okra variety were sown in a 5L plastic bucket, each treatment was replicated five times. Seedlings were thinned to one plant per stand four weeks after sowing. The growing plants were adequately watered at an interval of three days. At the stage of flower and fruit production, the leaf length, leaf width, plant height, length of leaf stalk, leaf stalk girth, stem girth were measured with a tape graduated in centimeters. Stem thickness was measured with meter rule and the number of leaves per stand was counted. Pods were harvested at full maturity and the pod length was measured using a tape graduated in centimeters. Pod thickness was measured with meter rule; the number of seeds per pod was counted. Description: Pod weight and seed weight were taken using a weighing balance. Data collected were subjected to analysis of variance (ANOVA) and means were separated using the Duncan multiple range test (DMRT). Seven (7) of the eleven characters investigated were found to be significantly different that is they are not stable while the remaining four (4) has no significant difference indicating their stability 2015-10-01T00:00:00Z